PRODUCTION OF ALPHA TOXIN OF CLOSTRIDIUM PERFRINGENS
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چکیده
منابع مشابه
Nutritional Requirements of Clostridium Perfringens Pb6k for Alpha Toxin Production
A potent toxin was produced by a strain of Clostridium perfringens PB6K in a chemically defined medium consisting of 19 amino acids, fructose, vitamins and minerals. A large amount of L-arginine (10 mg/ml or higher concentration) was required for toxin production. An excess of L-cystine (0.1 mg/ml or higher) reduced the yield of toxin seriously. Zinc and manganese, though ineffective for growth...
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The Gram-positive anaerobic bacterium Clostridium perfringens is widely distributed in nature, especially in soil and the gastrointestinal tracts of humans and animals. C. perfringens causes gas gangrene and food poisoning, and it produces extracellular enzymes and toxins that are thought to act synergistically and contribute to its pathogenesis. A complicated regulatory network of toxin genes ...
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Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding component). Ib binds to the surface receptor of target cells and translocate Ia into the cytosol of cells. The aim of this study was to clone toxigenic epi...
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Clostridium perfringens alpha-toxin is a key mediator of gas gangrene, which is a life-threatening infection that manifests as fever, pain, edema, myonecrosis, and gas production. Alpha-toxin possesses phospholipase C and sphingomyelinase activities. The toxin is composed of an N-terminal domain (1-250 aa, N-domain), which is the catalytic site, and a C-terminal domain (251-370 aa, C-domain), w...
متن کاملProduction of alpha toxin of Clostridium perfringens. II. Failure to produce a potent alpha toxin in the simplified media.
production of alpha toxin. Various kinds of enzymatic digests of protein have been used as a source of •gtoxin toxin factor•h (Adams et al., 1945; Logan et al., 1945; van Heyningen, 1948). Adams, Hendee and Pappenheimer (1947) showed that at least two factors present in the enzymatic digests of certain proteins and extracts of pancreas were necessary for the maximum production of alpha toxin in...
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ژورنال
عنوان ژورنال: Japanese Journal of Medical Science and Biology
سال: 1956
ISSN: 0021-5112,1884-2828
DOI: 10.7883/yoken1952.9.81